Scienceline Publications Journal of World's Poultry Research 2322-455X 6 1 2016 March Evaluation of the Nutritive Value and Detection of Contaminants in Feed and Water Samples in Open Poultry Houses of Layer Farms in Gezira State, Sudan 18 24 http://jwpr.science-line.com/attachments/article/35/J%20World%20Poult%20Res%206(1)%2018-24,%20%20March%2025,%202016.pdf EN Nura EL-Dikeir Department of Animal Production, Faculty of Agricultural Sciences, Dallanj University, Sudan Mohamed Yousef Mohamed Elbeeli Department of Animal Production, Faculty of Agricultural Sciences, University of Gezira, Sudan Awad Mohamed Abdel-Rahim Department of Microbiology, Faculty of Technology, University of Gezira, Sudan Neama Adam Eshag Shagra University, Faculty of Science and Humanities, Quiayhai, Kingdom of Saudi Arabia Sayda Ahmed Mohamed Ali Department of Animal Production, Faculty of Agricultural Sciences, University of Gezira, Sudan This study was carried out in Gezira state, Sudan to investigate feed and drinking water composition and contamination in open layer houses. Data was collected through individual interviews (questionnaires) of 97 randomly selected poultry farm owners during farms visits. Water and feed samples were collected from 20% of the visited farms and data was analyzed using SPSS. Results indicated that feed samples obtained from farms and mills had higher levels of crude protein than recommended; it was about (23-25%) in all localities, which affect birds’ kidney that negatively affect egg production. Metabolizable energy was in the recommended range in all localities. There was high fungal growth and aflatoxins presence in feeds in many localities. Total fungal count was from 113 colonies/g in Greater Medani municipality to 2850 colonies/gr in Almanagil municipality and aflatoxins were from 37.5% in Alkamleen to 66.7% in South of the Gezira and Greater Medani localities. Feed ingredients were also contaminated with fungal growth and aflatoxins presence. Drinking water indicated high pH and total hardness in many localities. There was also high bacterial total count in all localities and E-coli was from 5 colonies/ml in Alhasahesa municipality in the north to Greater Medani municipality mto150 colonies/ml in East of the Gezira locality. It was recommended that measures be taken to ensure poultry feed and drinking water safety in addition to adjusting feed composition to nutrients requirements for the specific production to sustain high productivity. Poultry feed Nutritive value Contaminants