Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. www.wvj.science-line.com;
Author: Hamouda AH, Sorour ShS, El-Habashi NM; El-Hussein AA (2018).
Date: 2018-09-16T13:12:39.527674+00:00

2018, Scienceline Publication

World’s Veterinary Journal

World Vet J, 8(1): 19-33, March 25, 2018

ISSN 2322-4568

Parasitic Infection with Emphasis on Tylodelphys spp. as

New Host and Locality Records in Nile Perch; Lates
niloticus from Lake Nasser, Egypt

Awatef Hamed Hamouda1*, Shimaa Sobhy Sorour2, Nagwan Mahmoud El-Habashi3 and El-Hussein Amar Adam4

1 Department of Fish Diseases, Faculty of Fisheries and Fish Technology, Aswan University, postal code: 81528, Egypt 2 Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Kafrelsheikh University, postal code: 33516, Egypt 3 Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, postal code: 33516, Egypt 4 Department of Aquatic Ecology, Faculty of Fisheries and Fish Technology, Aswan University, postal code: 81528, Egypt

*Corresponding author’s Email: awatefhamouda@yahoo.com

ABSTRACT

A total number of 200 Lates niloticus were collected alive from several and various localities at Lake Nasser in Aswan governorate, to investigate the prevailing parasites that infect this fish species. All the examined fish were positive for one or more parasites, three trematodes of two families were identified: Diplectanum simil e, O

Diplectanum lacustri s and Tylodelphys spp. (recorded for the first time in Lates niloticus representing new host and p

A

R
i

R
i
locality records), two nematodes of two families: Philometra ovata and L
c
e

:

G

3 larvae of Contracaecum spp.(has zoonotic
c
c

S
e
e

2

I
importance), one acanthocephalan parasite: Rhadinorhynchus niloticus, two crustaceans parasites of one family: p
iv 3

N
te e 2
d
d

2

A

Ergasilus kandti and Ergasilus latus, while no cestodal infections were recorded at all. The prevalence of trematodes

:

:

4

L

0

1

5
was at 95% meanwhile the nematodes were at 100% in addition to the acanthocephalan parasite was at 24.5% as 8

8

6

8

A

F

D

1

R
well, crustaceans parasites were at 69.5%. This study evaluated clinical signs, postmortem examinations, e
e

8
b
c

0

T
parasitological examinations, seasonal prevalence and histopathological investigations of infected fish in addition to 0

2

2

I

0

0

0

C

0
the relation between fish age and parasitism was also described. This study builds on our current understanding of 1

1

3

8

7

L

-8 E
different parasites infecting the wild Lates niloticus and provides novel information on the patterns of the isolated

parasites and also serves to reassure the consumers that the musculature (the edible part) of the fish was free from any parasitic infections and safe for human consumption provided that the fish must be eviscerated as soon as possible after being caught and adequately cooked.

Key words: Lates niloticus, Nile perch, Tylodelphys spp., Philometra ovata, Pathology, Lake Nasser INTRODUCTION

Fish is one of the most valuable sources of protein across the world as people obtain about 25% of their animal protein requirements from fish and shellfish (Matter et al., 2013). The rapid increase in the population in Egypt motivates the government to pay attention to the development of fisheries, especially in Lake Nasser. The production from such lake comprises a significant proportion of the Egyptian inland fisheries and is an important alternative source of protein in the face of increasing meat prices.

19

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

In Lake Nasser, the predominant species for sale as fresh fish are the tilapias, followed by Lates niloticus, which constitute 25.07% of the fish in the lake (GAFRD, 2016). The annual production of this fish in 2010 was 12,311 tons, representing 0.9% of the total annual production of the fish wealth sector in Egypt (GAFRD, 2010). In Egypt, the Nile perch ( Lates niloticus), commercially known as Samoos or Ishr-bayad, is a freshwater tropical, carnivorous fish of high commercial and recreational value in Africa, it inhabits a wide variety of habitats, including rivers, lakes, and irrigation channels (Aloo et al., 2017). Lates niloticus has been introduced to many lakes in Africa, including Lake Nasser where it is fished commercially. Lates niloticus has high edible white meat without bone rich in protein and vitamins especially omega-3 which is vital for human and considered a good fish for aquaculture development (Asnake, 2018).

In the last few years, the increase in parasitic infections among the fish of Lake Nasser has led to a drastic decrease in fish yields, low marketability, as well as rejection of the fish by consumers who fear the macroscopic parasites.

Moreover, some parasitic infections in the fish have zoonotic importance, delayed sexual maturity of the fish and increased fish mortality causing great economic losses (Noga, 2010 and Younis et al., 2017).

Trematodes, cestodes, acanthocephalans and nematodes had contributed the most among the major parasitic groups found in freshwater fish (Schmidt, 1990). Migrating larvae of Contracaecum and Philometra may cause tissue damage (Noga, 2010). Copepods are extremely important in aquatic ecosystems, where they constitute a food source for small fish, an intermediate host for fish parasites or fish parasites themselves, and serve as vectors of disease (Piasecki, 2004).

Due to the scarcity of scientific information on the incidence, characteristics and effects of parasitic infection on Lates niloticus in Lake Nasser, the present work aims to make an- up- to- date study that identifies the present status of some parasitic diseases afflicting Lates niloticus at the lake, the seasonal incidence, the histopathological alterations in the infected fish and analyze the relationship between fish age and each detected parasite.

MATERIAL AND METHODS

Ethical approval

Animal ethics committee, Faculty of Fish and Fisheries Technology, Aswan University, Egypt, approved the protocol and conducting of the study.

Study area

The High Dam Lake was created as a result of the construction of the Aswan High Dam in the 1960’s. The High Dam Lake extends for 480 kms, from the High Dam in Egypt to the Dal Cataract in Sudan, it lies300 km within the Egyptian borders (as Lake Nasser) and 180 km of it lies within the Sudanese borders (as Lake Nubia). Lake Nasser, together with Lake Nubia, is the second largest man-made lake in the world, after Lake Volta, in Ghana. Lake Nasser is now one of the most important sources of freshwater fish in Egypt.

Fish samples

A total number of 200 Lates niloticus of different weights and sizes (50 fish/ season) were collected, randomly and alive, from different localities of Lake Nasser at Aswan governorate during the period of February 2016 to January 2017.

The collected fish were transferred alive and kept in prepared glass aquaria in the laboratory of fish diseases, Faculty of Fisheries and Fish Technology, Aswan University. Identification of the host and the length frequency measurements were taken by measuring the standard length (the measurement from the most anterior tip of the body to the posterior end of the vertebral column), the total weight was taken in grams, and scale samples relevant to age determination were collected for subsequent examination.

Age determination

Scales of each fish were extruded from the area below the lateral line at a level behind the pectoral fin on the left side, and used to determine age according to Adam (2004). As the age distribution was determined, an age-length key was constructed. From this key, the length distribution, the number of fish belonging to each age group was determined.

All fish under study were belonging to age groups 1, 2, 3, 4, 5, 6, 7 and 8 years.

Clinical and postmortem examination

The fish were subjected to full clinical examination after that, they were euthanized rapidly by percussion stunning followed by destruction of the brain the procedures complied with local and national animal welfare laws, guidelines and policies, the external and internal gross lesions were recorded immediately according to the method described by Noga (2010).

20

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Parasitological examinations

Parasitological examinations were performed and then the collected parasites were washed, fixed, stained and permanently mounted according to the methods described by Lucky (1977) and Woodland (2006).

Identification of collected helminthes

The collected helminthes were identified according to the identification keys of Yamaguti (1958, 1961 and 1963); Thurston and Paperna (1969); Schaperclauus (1992) and Anderson (2000).

Histopathological examination

After necropsy, sections of gills, stomach, intestine, liver, and gas bladder were fixed immediately in 10% formalin and processed for histopathological evaluation, using the routine paraffin embedding method as described by Bancroft and Gamble (2007). Sections of 3μm thick were cut and stained using hematoxylin and eosin for light microscopic examination.

RESULTS

Population structure (age composition)

The frequency and age composition of Lates niloticus collected from the study area during the study period is presented in table 1. From the table, it is clear that age group 2 years old (55 %) of Lates niloticus was the most dominant group in the catch, followed by age group 1 (18.3 %), then age group 3 (17.8 %); the rest of the groups each comprise less than 8.9 %.

Table 1. Age–length key of Lates niloticus collected from lake Nasser, Aswan, Egypt showing frequency of fish specimens in different length groups and their distribution in each age group during the period of February 2016 to January 2017

Length groups

Age groups

Freq.

(mm)

1

2

3

4

5

6

7

8

200

3

3

0

0

0

0

0

0

0

230

7

7

0

0

0

0

0

0

0

260

23

17

6

0

0

0

0

0

0

290

28

4

24

0

0

0

0

0

0

320

39

0

38

1

0

0

0

0

0

350

22

0

14

8

0

0

0

0

0

380

18

0

5

12

1

0

0

0

0

410

5

0

3

2

0

0

0

0

0

440

6

0

2

3

1

0

0

0

0

470

4

0

1

2

1

0

0

0

0

500

5

0

0

2

3

0

0

0

0

530

1

0

0

0

0

1

0

0

0

560

1

0

0

0

0

1

0

0

0

590

0

0

0

0

0

0

0

0

0

620

0

0

0

0

0

0

0

0

0

650

3

0

0

0

0

0

3

0

0

680

1

0

0

0

0

0

0

1

0

710

1

0

0

0

0

0

0

1

0

740

2

0

0

0

0

0

0

1

1

Sum

169

31

93

30

6

2

3

3

1

Freq. = frequency

Clinical, postmortem and histopathological examinations induced by parasites

21

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Most of the examined fish showed no pathognomonic abnormalities except heavy parasitic infestation. Moreover, the examined fish exhibited restlessness, poor appetite, emaciation, slight abdominal distension as well as respiratory difficulties manifested by surface swimming and gasping.

Emaciation, dark or pale body coloration, detachment of scales and fin erosions were noticed, especially in the pelvic and caudal areas, together with hemorrhagic spots on different parts of the fish's body and excessive mucus.

Alternatively, paleness and congestion were observed in some parts of gills (Figure 1a and Figure 3a). Excessive mucous secretion and erosions of gills tips as well as white spots were observed (Figure 1a). Microscopically, gills revealed varying degrees of pathological changes including congestion of primary lamellae, interlamellar hyperplasia and adhesion of secondary lamellae, loss of the secondary lamellae in some sections (Figure 1b) and pillar cells hyperplasia at the tips of the primary lamellae (Figure 3d). Longitudinal sections of parasites including such crustaceans as Ergasilus spp., such as Ergasilus latus and Ergasilus kandti (Figures 1e and 1f), and monogenea spp. (Figure 3d) such as Diplectanum simile and Diplectanum lacustris were also observed.

The internal organs of naturally infected fish were pale and anemic, with enlargement and congestion of the spleen and liver (Figure 2a), together with congested and hemorrhagic gas bladder (Figure 4a), hemorrhage of the stomach as well as the enteritis was observed, especially in high parasitic load. Visible parasites could be seen by the naked eye (Figures 2a and 2b). The stomach and intestine revealed marked necrosis, sloughing of the covering epithelium and infiltrations of inflammatory cells, including mononuclear cells and heterophils in the mucosa and submucosa (Figures 6c, 6d, 6e and 6f).

Moreover, encysted third stage larva of Contracaecum spp. was observed microscopically attached to the serosa of the stomach, together with the significant inflammation of its wall (Figure 2f). The gas bladder revealed hemorrhage and inflammation but the identified parasites, either nematode, or trematode, were not detected microscopically. The livers of the infected fish revealed marked congestion, fatty change and multifocal necrosis of the hepatocytes, which in some areas had infiltrated with mononuclear cells and heterophils (Figures 6a and 6b). Marked heterophils infiltrations were also observed. Proboscis of acanthocephalan was observed and surrounded with inflammatory cells including mononuclear cells and heterophils (Figure 5f).

Parasitological examinations

On the bases of the morphological examinations, the following parasites were identified from Lates niloticus: Diplectanum simile and Diplectanum lacustris (Figures 3b and 3c) as monogeneans trematodes microscopically exhibit a wide range of shapes and sizes as well as Ergasilus kandti and Ergasilus latus (Figures 1c and 1d) as crustacean parasites from the gills.

Tylodelphys spp., un-encysted metacercariae digenetic trematodes, used fish as intermediate host was recovered from the wall of the gas bladder and reported as new host and locality records. The live specimens were white in color and the stained one had linguiform body, measuring few millimeters with rounded anterior end and conical pointed posterior end (Figures 4b, 4c, 4d, 4e and 4f).

Philometra ovata as nematodes isolated from the gas bladder measuring few millimeters and the live specimens were white in color and transparent (Figures 4g and 4h).

Third stage larva of Contracaecum spp. nematodes encapsulated in fibrin sheath and attached to the alimentary canal and encysted in the wall of the stomach in heavy infestation. The liberated live larvae of Contracaecum spp. were reddish-yellow in color with long, cylindrical body measuring 25-55 milimeters with rounded anterior and tapered posterior ends (Figures 2b, 2c, 2d and 2e).

Rhadinorhynchus niloticus, acanthocephalan isolated from the stomach, intestine especially rectum and attached to any organ in the abdominal cavity when liberated from the stomach and intestine. It was white in color with long, cylindrical body, measuring 2-3 centimeters and had a slender, hollow construction proboscis that forms the anterior end (Figures 5a, 5b, 5c, 5d and 5e).

22

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Figure 1. Gills of Lates niloticus from lake Nasser infected with Ergasilus spp. during the period of February 2016 to January 2017:

a. Pale gills of Lates niloticus showing excessive mucus and white dots ( Ergasilus spp. -arrow).

b. Gills of Lates niloticus showing interlamellar hyperplasia and adhesion of secondary lamellae, loss of the secondary lamellae (arrow), H&E,× 100.

c. Ergasilus latus infecting the gills of Lates niloticus, × 240.

d. Ergasilus kandti infecting the gills of Lates niloticus, ×200.

e, f. Gills of Lates niloticus showing longitudinal sections of Ergasilus latus and Ergasilus kandti as well as pillar cells hyperplasia at the tips of the primary lamellae, H&E, × 200

23

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Figure 2. Lates niloticus from lake Nasser infected with Contracaecum spp . during the period of February 2016 to January 2017:

a. Lates niloticus showing congested liver, gas bladder and stomach as well as visible third stage larvae of Contracaecum spp. attached to the alimentary canal.

b. Liberated third stage larvae of Contracaecum spp. recovered from their fibrinous sheaths.

c. Anterior end of third stage larvae of Contracaecum spp. (O, oesphagous- IC, intestinal caecum), ×200.

d. Anterior end of third stage larvae of Contracaecum spp., × 280.

e. Posterior end of third stage larvae of Contracaecum spp. (a, anal opening- t, tail end), ×200.

f. Stomach showing encysted third stage larva of Contracaecum spp. (arrow) as well as severe necrosis and infiltration of inflammatory cells in the lamina propria and submucosa, H&E, × 100

24

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Figure 3. Gills of Lates niloticus from lake Nasser infected with monogenetic trematodes during the period of February 2016 to January 2017:

a. Gills of Lates niloticus infected with Diplectanum spp. showing excessive mucus, pale altered with congested areas as well as erosions of the gills tips.

b. Wet mount of Diplectanum simile infecting the gills of Lates niloticus, ×200.

c. Diplectanum lacustris infecting the gills of Lates niloticus stained with acetic acid alum carmine, ×500.

d. Gills of Lates niloticus showing pillar cells hyperplasia(arrow)at the tips of the primary lamellae as well as
monogenea spp., H&E,×200

25

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Figure 4. Gas bladder of Lates niloticus from lake Nasser infected with Tylodelphys spp. and Philometra ovata during the period of February 2016 to January 2017:

a. Gas bladder of Lates niloticus infected with Tylodelphys spp. and Philometra ovata showing slightly congested and hemorrhagic areas.

b. Wet mount of Tylodelphys spp. recovered from the gas bladder of Lates niloticus, ×200.

c,d,e, f. Tylodelphys spp. recovered from the gas bladder of Lates niloticus stained with acetic acid alum carmine,×200.

(OS, oral sucker - PS, pseudosucker - PH, pharynx- O, oesophagus - IC, intestinal caeca - AC, acetabulum -EB, excretory bladder - HF, holdfast).

g. Anterior end of Philometra ovata recovered from the gas bladder of Lates niloticus (P, papillae - O, oesophagus - IC, intestine), ×4000.

h. Posterior end of male Philometra ovata (SP, spicules - G, gubernaculum) recovered from the gas bladder of Lates niloticus, × 4000

26

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Figure 5. Rectum, stomach and liver of Lates niloticus from lake Nasser infected with Rhadinorhynchus niloticus during the period of February 2016 to January 2017:

a. Rhadinorhynchus niloticus inserted their proboscises in the congested rectum of Lates niloticus.

b. Rhadinorhynchus niloticus inserted its proboscis in the stomach of Lates niloticus (arrow).

c. Anterior end of Rhadinorhynchus niloticus infecting Lates niloticus (Pr, Proboscis - I, intestine), ×40.

d. Proboscis of Rhadinorhynchus niloticus infecting Lates niloticus showing the arranged hooks (H) (arrows), × 400.

e. Middle and posterior parts of Rhadinorhynchus niloticus infecting Lates niloticus (T, tests - CG, cement gland - SV, seminal vesicle - GP, genital pore), ×40.

f. Liver of Lates niloticus showing proboscis (arrow) of acanthocephalan surrounded with inflammatory cells including 27

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

mononuclear cells and heterophils, H&E,× 200

Figure 6. Liver, stomach and intestine of Lates niloticus from lake Nasser infected with Contracaecum spp. during the period of February 2016 to January 2017:

a. Liver showing marked necrosis of hepatocytes (arrow), H&E, × 100.

b. Liver showing fatty change and marked heterophils infiltrations (head arrow), H&E, × 200.

c. Stomach showing severe necrosis of the mucosa (asterisk) and infiltration of inflammatory cells in lamina propria and submucosa, H&E, × 100.

d. Higher magnification of Figure 6c.

Stomach showing marked heterophils infiltrations (arrow) in the lamina propria, H&E, × 200.

e. Intestine showing severe necrosis of the mucosa and infiltration of inflammatory cells in the lamina propria and submucosa, H&E, × 100.

f. Higher magnification of Figure 6e, intestine showing marked heterophils and mononuclear cells infiltrations in the 28

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com
lamina propria, H&E, ×200

The total and the seasonal prevalence of detected parasites

The examined fish; Lates niloticus revealed a trematodal, nematodal, acanthocephalan and crustaceans infection rates of 95%, 100%, 24.5% and 69.5% respectively. However, there were no cestodal infections along the period of study, as presented in (Tables 2 and 3).

Concerning the seasonal dynamics of external Diplectanum simile and Diplectanum lacustris isolated from the gills of Lates niloticus with an overall prevalence of (30%), a higher infection rate was observed during autumn (50,58%), followed by summer (40,45%), spring (20,33%) and then winter (10, 18%) respectively, as presented in (Table 4). The incidence and intensity of infection with Diplectanum spp. in Lates niloticus was highest in the class 1 age group.

Tylodelphys spp . , un-encysted metacercaria isolated from the gas bladder, used Lates niloticus as an intermediate host with a total prevalence of (89%). A higher infection rate was observed during summer (96%), followed by autumn (90%), spring (86%) and then winter (84%), as described in (Table 4). The incidence and intensity of infection with Tylodelphys spp. in Lates niloticus increased with the class 2 age group.

Regarding the prevalence of different species of nematodal infections in Lates niloticus, Philometra ovata in the gas bladder was recorded with a total prevalence of 12.5 % and was higher in summer (20%) then spring (12%), winter (10%) and autumn (8%) (Table 4). It was observed much more in individuals belonging to the class 3 age group.

The third stage larvae of Contracaecum spp. in the abdominal cavity of the fish investigated were recorded with a prevalence rate of 100% throughout the period of study (Table 4). The recorded presence of Rhadinorhynchus niloticus isolated from the stomach and intestine of Lates niloticus was highest in winter (40%), then spring (30%), summer (20%) and autumn (8%) with a total prevalence of 24.5 % (Table 4).

The presence of nematodes ( Contracaecum spp. and Philometra ovata) and the acanthocephalan ( Rhadinorhynchus niloticus) in Lates niloticus had increased in incidence and intensity with the age of fish. Ergasilus kandti and Ergasilus latus were found in the gills of investigated fish at a higher infection rate in summer (90%, 92%), then spring (86%, 90%), autumn (82%, 87%) and winter (20%, 30%) respectively (Table 4). The incidence and intensity of infection with Ergasilus spp. in Lates niloticus was highest in the class 1 age.

In the present study, we noticed that while the gills are heavily infested with Ergasilus spp., the number of Diplectanum spp. drastically decreases or are even entirely absent. Interestingly, the musculature of Lates niloticus was completely free from any larvae, encysted meatacercariae and adult worms.

The parasites detected in Lates niloticus were found throughout the period of study. Some of them showed clear seasonal variation while the others were of nearly uniform frequency during the different seasons, varying only in the intensity of infections. The detected parasites were identified in their larval stages and adult worms were also reported.

DISCUSSION

Signs found on Lates niloticus infected with different parasites may be explained as sequences of parasites presenting different parts of the fish. Similar results have been reported on other fish hosts and other parasitic diseases (Hamouda, 2014). Few studies have reported on the occurrence of third stage larvae of Contracaecum spp. in Lates niloticus of Lake Nasser without any behavior abnormalities (Abd Alkareem, 2004).

Paleness and congestion in some parts of gills might be attributed to the mechanical injuries caused by

Diplectanum spp. and Ergasilus spp. at the onset of infection leading to congestion. Indeed, when flukes increased in number and attacked the gills aggressively, they increase their feeding activities on the blood of the highly vascularized gills, causing anemia and pallor of the gills in the chronic phase. Excessive mucous secretion (as a defensive mechanism to diminish the irritant effect of the pathogen) and the white structures in the gills were Ergasilus spp. These results were nearly similar to those recorded by Noga (2010); Rashed (2013) and Hamouda (2014).

Internal gross lesions induced by parasites may be attributed to the presence of macroscopic parasites of third stage larvae of Contracaecum spp., Philometra ovata, Tylodelphys spp. and Rhadinorhynchus niloticus (which embed themselves in the lining mucosa of the stomach and intestine, causing local damage and possibly peritonitis). Moreover, proteolytic enzymes discharged from adult worms may be degrading the gastric and intestinal tissues (Woo, 1995).

Helminthes produce toxic metabolic by-products which harm the infected host by causing occlusion of blood vessels, intestine and other ducts and resulting in the inflammation and congestion described in the internal organs. The above-mentioned reports suggesting the mechanism of tissue damage induced by parasites were confirmed histopathologically.

However, no study has described in detail signs, lesions, evidences and histological damages induced by parasites in Lates niloticus as the present paper.

29

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Latus niloticus is a carnivorous fish that assists in the greater transmission of parasites through feeding on young infested fish or aquatic animals that harbor it in the infective stages. This could be the reason for the maximum parasitic infestation rate (100%).

There is scarcity of information on the isolated parasites of Lates niloticus from lake Nasser except 3rd stage larvae of Contracaecum spp. (Younis et al., 2017) and Rhadinorhynchus niloticus.

The metacercariae of Tylodelphys spp . , are highly active and never encyst (Schaperclaus, 1992). Many authors around the world have detected Tylodelphys spp. in the vitreous humor of fish other than Lates niloticus (Otachi, 2009; Blasco-Costa et al., 2017 and Chaudhary et al., 2017) and from cranial cavity of Clarias gariepinus (Chibwana et al.

2015). But to our knowledge there is no record of isolation of such parasites from the swim bladder and from Lates niloticus so it is new host and locality records.

Philometra ovata was recorded with a total prevalence of 12.5 % and was highest in summer (20%) then spring (12%), winter (10%) and autumn (8%). It was observed much more in individuals belonging to the class 3 age group.

These results were nearly identical with those recorded by Innal and Keskin (2005) on Leucis cephalus L. fish and lower than recorded by YI-TE L (2014) on European minnows. Unidentified species of Philometroides were recorded from Lates niloticus in Egypt by El-Nafar et al . (1983) with a prevalence of 1.3%. Philometra lati isolated from the abdominal cavity of Lates niloticus and Philometra spiriformis sp. n. from capsules on the inner surface of the gill covers of L.

niloticus were recorded by Moravec et al . (2009) in Kenya. Members of the family Philometridae has been recorded in different fish species other than Lates niloticus in Africa (Khalil, 1960, 1965, 1969 and 1973; Fahmy et al., 1976; El-Nafar et al., 1983 and Moravec and Van As, 2001).

The third stage larvae of Contracaecum spp. were recorded with a prevalence of 100% throughout the period of study. This result was identical with that recorded by Rabei (2009) and Younis et al. (2017) and this may be explained as the definitive hosts of Contracaecum spp. are pelicans, cormorants and herons and these are ubiquitous throughout the year around lake Nasser, so the infection was equally throughout the year. Contracaecum spp. larvae belonging to Anisakid nematodes which are considered to be responsible for anisakidosis disease, a serious zoonotic disease, and there has been a dramatic increase in its reported prevalence throughout the world in the last two decades (Lymbery and Cheah, 2007) so if the fish are not frozen or filleted soon after capture, larval nematode may migrate into the flesh.

Accordingly, humans may be infected if viable larvae are consumed in uncooked or undercooked fishes (Younis et al., 2017).

Rhadinorhynchus niloticus was highest in winter (40%), then spring (30%), summer (20%) and autumn (8%) with a total prevalence of 24.5%. This was higher than that recorded by Ebraheem (1992) and Abd Elmageed (2015). The presence of nematodes ( Contracaecum spp. and Philometra ovata) and the acanthocephalan ( Rhadinorhynchus niloticus) in Lates niloticus increased in incidence and intensity with the age of fish and this may be owing to the accumulation of the worms over a longer period giving them available spaces for colonization and accumulation in addition to the larger size of fish, tend to be too big for the piscivorous bird to feed upon (Gichohi et al . , 2008 and Zekarias and Yimer, 2008).

Ergasilus kandti and Ergasilus latus were found in the gills of investigated fish at a higher infection rate in summer (90, 92%), then spring (86, 90%), autumn (82, 87%) and winter (20, 30%), respectively. This was nearly similar to that recorded by Paperna (1968) but, Aladetohun et al. (2013) recorded the highest infestation rate of Ergasilus spp. in Mugil cephalus in the rainy seasons.

In the present study, we noticed that while the gills are heavily infested with Ergasilus spp., the number of Diplectanum spp. is drastically decreased or are even entirely absent. This may be because the heavy infestations with Ergasilus make the gills less suitable for monogeneans to establish themselves (Tiiurston and Paperna, 1969). Ergasilus feeding activity induces severe focal damage and very heavy infestations can be lethal (Noga, 2010).

The parasites detected in Lates niloticus were found throughout the period of study. Some of them showed clear seasonal variation while the others were of nearly uniform frequency during the different seasons, varying only in the intensity of infections. The snail is restricted to waters that remain warm (probably >17 ° C) year – round ( Noga, 2010) and in Aswan, the high temperature in all seasons is maintained nearly throughout the year. This favors the development of snails, invertebrate hosts and the final hosts (fish, crocodiles, frogs, snakes and aquatic birds) as well as, an increase in plankton production, which is the source of nutrition of intermediate hosts.

The detected parasites were identified in both larval stages and adult worms, indicating that Lates niloticus act as intermediate, definitive or paratenic host contributing the expansion of infestations on intra or inter specific hosts. This could be due to the nature of the life cycle in these parasites, which involves the presence of snails or invertebrate (a crustacean; sometimes an annelid, coelenterate and mollusk) as first intermediate hosts, followed by fish as second intermediate host and finally aquatic birds, fish and reptiles as definitive final hosts (Syobodova and Kolarova, 2004).

30

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Our results may differ partially or completely with many authors and this may be attributed to the different localities of the examined fish, type, age and sex of fish examined as well as, water hydrochemistry in each locality (Hamouda, 2014).

31

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Table 2. Prevalence of trematodes and nematodes among the examined Lates niloticus in lake Nasser, Egypt during the period of February 2016 to January 2017

Trematodal infections

Nematodal infections

Fish

No.

Total Diplectanum

Total of trematodal

Total of nematodal

Diplectanum simile

Diplectanum lacustris

Tylodelphys spp.

Contracaecum spp.

Philometra ovata
species

Exam.

infections
infections
infections

Fish

Infected

% of

Infected

% of

Infected

% of

Infected

% of

Infected

% of

Infected

% of

Infected

% of

Infected

% of

No.

infections

No.

infections

No.

infections

No.

infections

No.

infections

No.

infections

No.

infections

No.

infections

Lates

200

33

16.5

45

22.5

60

30

178

89

190

95

200

100

25

12.5

200

100
niloticus

No. exam. = Number of examined

Table 3. Prevalence of acanthocephalans, crustaceans and cestodes among the examined Lates niloticus in lake Nasser, Egypt during the period of February 2016 to January 2017

Rhadinorhynchus niloticus

Ergasilus spp.

Cestodes

Fish species

Ergasilus kandti

Ergasilus latus

Total Ergasilus infections

No. exam. Fish

Infected No.

% of infections

Infected No.

% of infections

Infected No.

% of infections

Infected No.

% of infections

Infected No.

% of infections

Lates niloticus

200

49

24.5

60

30

90

45

139

69.5

0

0

No. exam. = Number of examined

Table 4. The seasonal prevalence, infection, intensity and mean/fish of helminth parasites in different organs of Lates niloticus from lake Nasser, Egypt during the period of February 2016 to January 2017

Seasonal prevalence

Type of parasite

% of infected fish

Susceptible organs

No. of Parasites per inf. fish

Mean /fish

Spring

Summer

Autumn

Winter

Diplectanum simile

16.5

20

40

50

10

Gills

4-10

4

Diplectanum lacustris

22.5

33

45

58

18

Gills

6-20

7

Tylodelphys spp .

89

86

96

90

84

Gas bladder

3-33

20

Philometra ovata

12.5

12

20

8

10

Gas bladder

6-60

28

Contracaecum L3 larvae

100

100

100

100

100

Associated with the alimentary canal

1-99

33

Rhadinorhynchus niloticus

24.5

30

20

8

40

Stomach and intestine

1-7

4

Ergasilus kandti

30

86

90

82

20

Gills

10- 40

15

Ergasilus latus

45

90

92

87

23

Gill

10-60

22

Cestodes

0

0

0

0

0

ـ ـ ـ ـ ـ

0

0

No. = Number; inf. = infected

32

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33.

http://wvj.science-line.com

CONCLUSION

Parasitic infestation of Lates niloticus is an important factor affecting wild populations of Lates niloticus and another aquatic species in Lake Nasser. Therefore, extended investigations about the effects of parasitic infection on fecundity, survival of this fish species are recommended to ensure successful fishery management plans.

Lates niloticus must be gutted soon after capture to avoid the attack of the detected parasites to its musculature which is completely free from any parasitic infection. The disposal of viscera or infected fish parts in water should be strenuously prohibited. Regular monitoring of the fish in Lake Nasser is a must. Further studies on Tylodelphys spp . in Lates niloticus (new host and locality records) are needed and urged.

DECLARATIONS

Author’s contribution

All authors contributed equally to this work whereas they designed, conducted the research and wrote the manuscript.

Competing interests

The authors have declared that no competing interest exists.

REFERENCES

Abd Alkareem O (2004). Studies on round worms infection in freshwater fishes in Lake Nasser. Ph.D. Thesis, Faculty of Veterinary Medicine, Cairo University, Egypt.

Abd Elmageed A (2015). Biological and taxonomical studies on some parasites infecting commercial fishes in Egypt. Ph.D. Thesis, Faculty of Science, South Vally University, Egypt.

Adam A (2004). Stock assessment of some important commercial fish species of Lake Nasser, Egypt. Ph.D. Thesis, Faculty of Science, Assiut University, Egypt.

Aladetohun NF, Sakiti NG and Babatunde EE (2013). Copepoda parasites in economically important fish, Mugilidae ( Mugil cephalus) and Liza falcipinnis from Lac Nokoue Lagoon in Republic of Benin, West Africa. Journal of Environmental Science and Technology, 7: 799-807.

Aloo PA, Njiru J, Balirwa JS and Nyamweya CS (2017). Impacts of Nile perch, Lates niloticus, introduction on the ecology, economy and conservation of Lake Victoria, East Africa. Lakes and Reservoirs: Research and Management, 22: 320–333.

https://doi.org/10.1111/lre.12192

Anderson RC (2000): Nematode Parasites of Vertebrates: Their Development and Transmission, 2nd Edition. CABI publishing, UK.

DOI /10.1079/9780851994215.0000

Asnake W (2018). Nile Perch ( Lates niloticus): The Promising White Meat of the World. Journal of Nutrition & Food Sciences, 8(2): 680. DOI: 10.4172/2155-9600.1000680

Blasco-Costa, Poulin R and Presswell B (2017). Morphological description and molecular analyses of Tylodelphys sp. (Trematoda: Diplostomidae) newly recorded from the freshwater fish Gobiomorphus cotidianus (common bully) in New Zealand. Journal of Helminthology, 91, 332–345. DOI: 10.1017/S0022149X16000298

Bancroft JD and Gamble M (2007). Theory and Practice of Histological Techniques. 5th edition; Churchill Livingstone, London, UK, pp. 125-138. ISBN-10 0-44-306435-0 ISBN-13: 978-0443064357

Chaudhary A, Gupta S, Tripathi R and Singh HS (2017). Morphological and molecular analyses of Tylodelphys spp. metacercaria (Trematoda: Diplostomidae) from the vitreous humour of two freshwater fish species, Channa gachua (Ham.) and Puntius sophore (Ham.). Veterinary Parasitology, 244 (2017): 64–70. DOI: 10.1016/j.vetpar.2017.07.016. Epub 2017 Jul 21.

Chibwana FD, Nkwengulila G, Locke SA, McLaughlin JD and Marcogliese DJ (2015). Completion of the life cycle of Tylodelphys mashonense (Sudarikov, 1971) (Digenea: Diplostomidae) with DNA barcodes and rDNA sequences. Parasitology Research, 114:3675–3682. DOI: 10.1007/s00436-015-4595-8. Epub 2015 Jul 1.

Ebraheem ME (1992). Studies on some of the parasites in some Nile fishes in Sohag Governorate. Ph.D. Thesis, Faculty of Science, Sohag, Assuit University, Egypt.

El-Nafar MK, Saoud MF and Hassan IM (1983). A general survey of the helminth parasites of some fishes of Lake Nasser at Aswan, A. R. Egypt. Assiut Veterinary Medical Journal, 11: 141–183.

Fahmy MAM, Mandour AM and EL-Nafar MK (1976). On some nematode parasites from the freshwater fishes in Assiut Province, Egypt. Veterinary Medical Journal, (Egypt) 24: 263–276.

GAFRD (2010). General Authority for Fishery Resources Development. Fish Statistics book 2010, Ministry of Agriculture and Land Reclamation, Egypt.

GAFRD (2016). General Authority for Fishery Resources Development. Inland lakes; Lake Nasser 2014 reprinting in 2016. Ministry of Agriculture and Land Reclamation, Egypt.

33

To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Gichohi CM, Mbuthia PG, Waruiru RM, Ngatia TA, Maingi N, Weda EH and Otieno R (2008). Preliminary study of the prevalence of helminths and their associated pathological lesions in four fish species from River Tana. Bulletin of Animal Health Production of Africa, 56: 38 – 45. http://dx.doi.org/10.4314/bahpa.v56i1.32824

Hamouda AH (2014). Studies on the trematodal and cestodal diseases in cultured freshwater fish in Kafr El-Sheikh Governorate. Ph.

D. Thesis, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt.

Innal D and Keskin N (2005). Philometra ovata (Zeder, 1803) (Philometridae) in European chub ( leuciscus cephalus L., 1758) living in Camkoru Lake (Camlidere-Ankara). Journal of Animal and veterinary Advances, 4:959-961.

Khalil LF (1960). On a new nematode, Nilonema gymnarchi gen. et sp. nov., (Dracunculidae), from a freshwater fish in the Sudan.

Journal of Helminthology, 34: 55–58. https://doi.org/10.1017/S0022149X00020344

Khalil LF (1965). On a new philometrid nematode, Thwaitia bagri sp. nov., from a freshwater fish in the Sudan. Journal of Helminthology, 39:309–312. https://doi.org/10.1017/S0022149X00020733

Khalil LF (1969). Studies on the helminth parasites of freshwater fishes of the Sudan. Journal of Zoology, London 58: 143–170.

https://doi.org/10.1111/j.1469-7998.1969.tb02132.x

Khalil LF (1973). Some nematodes from the freshwater fishes of Rhodesia with the description of a new species Cithariniella petterae n. sp. Annales de parasitology humaine et comparée, 48: 811–818. https://doi.org/10.1051/parasite/1973486811

Lucky Z (1977). Methods for the diagnosis of fish diseases. Amerind publishing Co, PTV. LTD, New Delhi, Bombay, Calcutta and New York. ISBN 10: 0862493552 ISBN 13: 9780862493554

Lymbery A and Cheah FY (2007). Anisakid nematodes and anisakiasis. In: K.D. Murrell & B. Fried, eds. Food-borne Parasitic Zoonoses, pp. 185–207. Springer, New York, USA. ISBN-13: 978-0-387-71357-1 e-ISBN-13: 978-0-387-71358-8

Matter AF, Abbass AA, Abd El Gawad EA, El –Asely AM and Shaheen AA (2013). Studies on myxosporidiosis in some freshwater fishes. Benha Veterinary Medical Journal, 25: 316-325.

Moravec F, Charo-Karisa H and Jirků M (2009). Philometrids (Nematoda: Philometridae) from fishes of Lake Turkana, Kenya, including two new species of Philometra and erection of Afrophilometra gen. n. Folia Parasitologica, 56: 41–54. ISSN 0015-5683 (print), ISSN 1803-6465 (online)

Moravec F and Van AS JG (2001). Philometroides africanus sp. n. (Nematoda: Philometridae), a new tissue parasite of the African pike Hepsetusodoe (Pisces) in Botswana. Folia Parasitologica, 48:127–131.

Noga EJ (2010). Fish Diseases, Diagnosis and Treatment. 2nd Edition, Iwa State University, Press, Ames. ISBN-13: 978-0-8138-0697-6/2010.

Otachi E (2009). Studies on occurrence of protozoan and helminth parasites in Nile tilapia ( Oreochromis nilotica, L.) from Central and Eastern Provinces, Kenya. M. Sc. Thesis, Egerton University, Nakuru, Kenya.

Paperna I (1968). Ectoparasitic infections of fish of Volta Lake, Ghana. Bulletin of the Wildlife Disease Association, 4: 135–137.

https://doi.org/10.7589/0090-3558-4.4.135

Piasecki, Wojciech, Goodwin, Andrew E, Eras, Jorge C, Nowak and Barbara F (2004). Importance of Copepoda in Freshwater Aquaculture, Zoological studies, 43:193-205.

Rabei GM (2009). Light and Scanning Electron Microscopy studies on Contracaecum sp. larvae and Cithariniella citharini (Nematoda) from Lake Nasser fishes. M. V. Sc. Thesis, Faculty of Science, Aswan, South Valley University, Egypt.

Rashed MA (2013). Studies on some parasitic diseases on the gills of some freshwater fishes. Ph. D. Thesis, Kafr El-Sheikh University, Egypt.

Syobodova Z and Kolarova J (2004). A review of the diseases and contaminant related mortalities of tench ( Tinca tinca L.).

Veterinarni Medicina. Czech, 49:19-34.

Schaperclaus W (1992). Fish diseases Volume (II). A. A. Balkema, Roterdam, Berlin. ISBN 9061919525

Schmidt GD (1990). Essentials of Parasitology (4th Edition). W. M. C. Brown Publishers.

Thurston JP and Paperna I (1969). Diplectanum lacustris sp. nov. (Dactylogyroidea: Diplectanidae), a monogenetic trematode from the gills of the Nile perch. Proceedings of the Helminthological Society of Washington, 36:214-218.

Woo PTK (1995). Fish diseases and disorders. Volume І: Protozoan and Metazoan infections, CAB International, Wallingford, Oxon, U.K.

Woodland J (2006). National Wild Fish Health Survey – Laboratory Procedures Manual. 3.1 Edition. U.S. Fish and Wildlife Service, Pinetop, AZ.

Yamaguti S (1958). Systema Helminthum. Vol. I. The digenetic trematodes of the vertebrates. Parts I and II. Inter science Publication, New York.

Yamaguti S (1961). Systema Helminthum Vol. III, parts 1 and 2. The nematodes of vertebrates. Interscience. Publication, New York.

Yamaguti S (1963). Parasitic copepod and branchiura of fishes. Interscience Publication, New York.

Younis AE, Saad AI and Rabei JM (2017).The occurrence of Contracaecum sp. larvae (Nematoda: Anisakidae) in four teleostean species from Lake Nasser, Egypt: morphological and molecular studies. The Journal of Basic and Applied Zoology, 78:9. DOI 10.1186/s41936-017-0012-4

YI-TE L (2014). Influences of the parasitic nematode Philometra ovata on European minnows. Dissertations in Frostry and Natural Science No. 168, University of Eastern Finland, Department of Biology. ISBN: 978-952-61-1641-9

Zekarias T and Yimer T (2008). Study on parasites of fish at Lake Awassa, Ethiopia. Bulletin of Animal Health and Production in Africa, 55: 151 – 155. http://dx.doi.org/10.4314/bahpa.v55i3.32803

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To cite this paper: Hamouda AH, Sorour ShS, El-Habashi NM and El-Hussein AA (2018). Parasitic Infection with Emphasis on Tylodelphys spp. as New Host and Locality Records in Nile Perch; Lates niloticus from Lake Nasser, Egypt. World Vet J, 8(1): 19-33. http://wvj.science-line.com

Document Outline

- Population structure (age composition)