Application of Different Hormonal Protocols for Improving Reproductive Performance of Barki Ewes. World Vet. J. 8(3): 55-64.;
Author: Ashour G, El-Bassiony MF, Dessouki ShM; El-Wakeel MA (2018).
Date: 2018-09-16T13:13:18.307731+00:00
Keywords: .

2018, Scienceline Publication

World’s Veterinary Journal

World Vet J, 8(3): 55-64, September 25, 2018

ISSN 2322-4568

Application of Different Hormonal Protocols for

Improving Reproductive Performance of Barki Ewes

Gamal Ashour1, Moharram Fouad El-Bassiony2*, Sherif Mohamed Dessouki1 and Mohamed Awad El-Wakeel2

1 Department of Animal Production, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt

2Animal and Poultry Physiology Department, Desert Research Center, 11753, Cairo, Egypt

*Corresponding author`s Email: moharramf@yahoo.com

ABSTRACT

This investigation was designed to assess reproductive improvement in Barki ewes using different hormonal protocols. Seventy-five non-pregnant and non-lactating Barki ewes were randomly assigned into three equal groups

(25 ewes each), namely G1, G2 and G3. (G1) served as control, while (G2) was treated with an intravaginal
progestagen impregnated sponge for 12 days then was removed. On the ninth day, all treated ewes received an

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intramuscular injection of prostaglandin F

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i

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i

2α. (G3) was also synchronized as G2, in addition to on the 10 th day ewes
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were received an intramuscular injection of 750 IU PMSG in descending doses for three days so that the last dose
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was injected at the 12th day concurrently with sponges' removal. Meanwhile, on the 14th day, all ewes in G3 were

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received an intramuscular injection of hCG hormone (500 IU/ewe). The results showed an increase in plasma

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progesterone level from the first day of pregnancy and rises up to the last day before parturition. Progesterone was

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found to be higher in G3 (that had higher twining rate) than in G2 than in G1. Insignificant increase in estrus

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y

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response was observed in groups (G2, G3) compared to G1 (100, 100 and 92%, respectively). Conception rate was

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significantly higher in G3 (100%) than G2 (92%) and G1 (88%). While, abortion rate was higher in G1 and

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surpassing G2 and G3 (9.0, 0.0 and 0.0 %, respectively). Lambing rate was significantly higher in G3 as compared to

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G2 and G1 groups (100, 92 and 80 %, respectively). While, weaning rate was recorded to be insignificantly higher in

G2 than in G1 and G3 (100.0, 95.45 and 94.29%, respectively). It could be concluded that, hormonal manipulation
using intravaginal progestagen impregnated sponge and PMSG in the presence of hCG; would be a proper way for
enhancing the reproductive efficiency of Barki ewes.

Key words: Barki ewes, Synchronization, Superovulation, Twining, Reproduction

INTRODUCTION

Low reproductive performance has been observed in sheep flocks raised traditionally in arid regions. This poor performance may be attributed to several factors including fertility, selection, nutrition and disease (Webb et al., 2004;

Yavuzer, 2005). Increasing Barki sheep productivity by increasing lambing frequency and fecundity is considered to be
the most important factor in the development of Barki sheep production. In this regard, increasing the rate of fecundity in
sheep offers the best opportunity to increase the efficiency of lamb meat production (Koyuncu and Alticekic, 2010).

Natural lambing in Barki sheep occurs throughout the year in a scattered manner negatively affecting survival and
growth rates of the lambs born during the unfavorable season of the year. Consequently, one of the most common ways
to alleviate problems related to disorderly lambing is the using of exogenous source of hormones for artificially controlling the time of mating (Mekuriaw et al., 2016). In addition, the use of hormonal manipulation for estrus synchronization creates the opportunity for timed breeding and lambing. This in turn results in taking the advantages of
seasonal variation of forage availability, photoperiod, labor resources and market demands. Estrus synchronization is a
valuable management tool that has been employed successfully in enhancing reproductive efficiency, particularly in
cows, ewes and does as early reported by Kusina et al. (2000). The success of a hormonal treatment for estrus synchronization depends on the productive status of ewes, season of the year, the type of hormonal protocol and the
method of administration. Multiple ovulation programs are also possible with the use of estrus synchronization for
improving herd productivity.

Estrus synchronization in small ruminants is achieved either by reducing the length of the luteal phase of the
estrous cycle with prostaglandin F2α or by artificially extending it with exogenous progesterone or potent progestagens

(Jainudeen et al., 2000 and Kusina et al., 2000). Synchronization of estrus has been practiced for the last few decades
when many protocols varying in degrees of success which based on the use of intravaginal progestagen devises inserted
for 7 to 14 days followed by gonadotropin injection at the time of devices' removal and introduction of teaser ram

55

To cite this paper: Ashour G, El-Bassiony MF, Dessouki ShM and El-Wakeel MA (2018). Application of Different Hormonal Protocols for Improving Reproductive Performance of Barki Ewes. World Vet. J. 8(3): 55-64.

Journal homepage: www.wvj.science-line.com

(Zarkawi, 2001). Also, hormonal treatments to control ovulation and reproduction are a perquisite for successful breeding and increasing the number of pregnant females (Motlomelo et al., 2002 and Husein et al., 2005). In small
ruminants, several programs have been used for improving fertility by controlling the ovarian activity. Acceptable results
of estrus responses have been achieved by using progestagen and equine Chorionic Gonadotropin (eCG) together at the
time of progestagen withdrawal (Fonseca et al., 2005; Husein et al., 2005).

Application of assisted reproductive technologies is a prerequisite for improving reproductivity and productivity of
livestock, especially in arid and semi-arid environments species which are usually characterized by low fertility. Most
recently, Kaya et al. (2018) noted that hormonal protocols are one of the main items to improve, herds productivity
including superovulation as proposed by Souza et al. (2014). In practice, generality of the superovulation protocols used
for embryo production in ewes, both in vivo and in vitro, consists of the administration of intravaginal progestagen impregnated sponges; both for synchronization of estrus and ovulation or for avoidance of spontaneous ovulation

(Cognie et al., 2003). The objectives of superovulation included inducing a high number of ovulations and subsequent
high fertilization rate, while at the same time ensuring a normal physiological environment in the reproductive tract for
embryo development. The application of superovulation technology to improve endogenous secretions of pregnant
hormones increases offspring productivity and gross revenue in the small scale farm (Andriyanto and Manalu, 2011).

The present study was conducted to place on record the lambing rates of ewes following and to investigate the
possibility of improving fertility and increasing twinning rate in Barki ewes under arid conditions of the North Western

Coast of Egypt using different hormonal protocols for estrus synchronization and superovulation.

MATERIALS AND METHODS

Experimental region

The present study was carried out during the period from June 2015 to January 2016 at Animal Production Unit,

Sustainable Development Center for Matrouh Resources, Matrouh Governorate, which belongs to the Desert Research

Center (DRC), Ministry of Agriculture and Land Reclamation. The station is located at 240 Km West of Alexandria and

222 Km Egyptian western boarders.

Ethical approval

This experiment was performed according to all ethics and animal rights (Desert Research Center). As much as this
work had considering all rules and regulations in conformity with the European union directive for the protection of
experimental animals (2010/63/EU).

Experimental animals, feeding and management

Seventy-five, non-pregnant and non-lactating, Barki ewes averaged 48.10±0.72 kg Live Body Weight (LBW) and
ranged between 2.5 and 3.5 years old were used in this study. All ewes were clinically examined for any reproductive
disorders as well as general health status. Vaccination against the major prevailing epidemic diseases, internal and
external parasites were controlled in proper time. Ewes were weighed before starting the experiment, and kept under an
intensive production system and housed in semi-open yards throughout the experimental period. Lambs were left all the
day time with their dams for suckling up to weaning age at three months of age. All groups were daily fed on a
concentrate (0.75 kg) and berseem hay (0.5kg) per head during the experimental period to cover their nutrient requirements during different physiological status according to Kearl (1982). Lambs were daily fed only on their dams’

milk from birth to weaning age at three months of age. The daily ration was offered in a certain mode of feeding starting
with concentrate mixture at 08:30 h followed by chopped rice straw at 12:00 h and continuing to the next morning
feeding. All animals drank fresh water three times a day (08:00, 14:00 and 20:00 h).

Experimental design

Animals were randomly divided into three equal groups (25 ewes per group (Figure 1). The first group (G1) was
served as a control without any hormonal treatment and was left without synchronization or superovulation. While, the
second group (G2) was treated using an intravaginal progestagen impregnated sponge (20 mg cronolone, Chronogest®

CR, product of intervet international B. V. and manufactured in the European Union, (EU) was inserted. Sponges
remained in situ for 12 days and were removed on the 12th day. On the ninth day, all ewes were received an intramuscular
injection of prostaglandin F2α (1 ml Synchromate each 1 ml solution containing 0.250 mg Cloprostenol, Bremer Pharma

GMBH, Germany). The third group (G3) was also synchronized using an intravaginal progestagen impregnated sponge
for 12 days and received an intramuscular injection of prostaglandin F2α, on the ninth day, while on the 10th day ewes of
the third group (G3) were received an intramuscular injection of 750 IU PMSG (Laboratorios Hipra, S.A. Avda. Ia Selva,

135, 17170 Amer (Girona) Spain). PMSG injections were applied in gradual decreasing doses for three days that each

56

To cite this paper: Ashour G, El-Bassiony MF, Dessouki ShM and El-Wakeel MA (2018). Application of Different Hormonal Protocols for Improving Reproductive Performance of Barki Ewes. World Vet. J. 8(3): 55-64.

Journal homepage: www.wvj.science-line.com

ewe received 275 IU at the 10th day, the second dose (250 IU) was injected at the 11th day, while the last dose (225 IU)

was injected at the 12th day at the time of sponges' removal. Meanwhile, on the 14th day, all ewes in G3 group received an
intramuscular injection of 500 IU/ewe of hCG hormone (Epifasi lyophilized ampoule contains 5000 I.U. of Human

Chorionic Gonadotrophin, hCG) and 10 mg of lactose, manufactured by Egyptian int. pharmaceutical industries Co.

(EIPICO, Egypt). During the synchronization period, all synchronized animals were subjected to a twice daily check

(morning and evening) to ensure that sponges remained in their position during the treatment period.

Figure 1. Experimental design and timeline for the treatment administration for the control (G1), synchronization (G2)

and synchronization + superovulation (G3) groups.

Blood sampling and progesterone assay

Blood samples (5 ml) were collected from the jugular vein into EDTA (ethylenediamine tetra-acetic acid)

containing tubes. Blood samples were collected immediately before sponge insertion on day 0 from ewes. The rest of the
samples were withdrawn on days 1, 3, 5, 7, 9, 10, 12, 13, 15, 17, 19, 30, 60, 90, 120, 150, 180 from all groups. Blood
plasma was pipetted into 1.5 mL Eppendorf tubes using sterilized plastic disposable Pasteur pipettes and then stored at

-20 ºC until assayed for (P4) concentration. Progesterone hormone was quantified by ELISA method using BIOS kit
provided by Chemux Bioscience Corporation, 385 Oyster Point Blvd Suite 5-6., South San Francisco, CA 94080, USA.

The standard curve ranged between 0-50 ng/ml. The sensitivity of the curve was 0.2 ng/ml.

Estrus detection and mating

Nine fertile Barki rams were introduced to the ewes of all experimental groups, three rams for each group for estrus
detection and mating; started 24 hours after removing the sponges (G2 and G3) and left to one week. While the control
group (G1), rams left with ewes from the zero day throughout two estrus cycles (35 days total). Rams were allowed to

57

To cite this paper: Ashour G, El-Bassiony MF, Dessouki ShM and El-Wakeel MA (2018). Application of Different Hormonal Protocols for Improving Reproductive Performance of Barki Ewes. World Vet. J. 8(3): 55-64.

Journal homepage: www.wvj.science-line.com
interchange among different ewe groups to avoid sire/group confounding effect. The fertile rams' chest was colored in a different color for each group.

Rams were introduced to ewes for seven days and left with them for estrus detection and natural mating. Estrus was
checked continuously by observation of the color marks on the ewe's rumps. Ewes were considered in estrus and mating
had occurred when the paint mark was heavy and evenly distributed or copulation was observed.

Reproductive parameters

The following traits were estimated for each of the treated groups:

• Estrus response = number of ewes showing signs of estrus / total ewes treated in each group ×100.

• Conception rate = number of ewes conceived / number of ewes showing estrus and mated in each group ×100.

• Lambing rate = number of ewes lambed / number of ewes mated in each group ×100.

• Weaning rate = number of lambs weaned / number of lambs born in each group ×100.

• Abortion rate = number of ewes aborted / number of ewes conceived in each group ×100.

• Litter size at birth = number of lambs born / number of ewe lambed.

• Litter size at weaning = number of lambs weaned / number of ewe lambed.

• Average litter weight at birth = the kilograms lambs born / number of ewe lambed.

• Fecundity rate = number of lambs born / number of ewe mated.

• Weaning weight = the kilograms lambs weaned / number of lambs weaned.

• Mortality rate up to weaning (%) =live lambs born - lambs weaned / live lambs born x100.

Statistical analysis

A General Linear Model procedure (SAS, 2004) was used for the statistical analyses of progesterone concentration,
milk yield and composition during the experimental period using the following model:

Yijk = µ + Pi + Tj + (P*T) ij + eijk

Where,

Yijk

= Any observation of kth animal within jth treatment within ith period
µ

= Overall mean

Pi

= Effect of ith period

Tj

= Effect of jth treatment (j = 1-3, 1= Tr1, 2= Tr2, 3= Tr3)

(P*T)ij

= The interaction between period and treatment
eijk

= Experimental error

Significant differences among means were detected using Duncan’s multiple range test (Duncan, 1955). While,
reproductive traits (estrus response, conception, lambing, weaning, fecundity, abortion, single, twining, triple, mortality
rates) and litter size at birth, litter size at weaning, weaning weight, sex ratio were analyzed using Chi-square test.

RESULTS AND DISCUSSION

Estrus response, and conception and abortion rates

The data presented in table (1) revealed that estrus response was non-significantly high in G2 (FGA+PGF2α) and

G3 (FGA+PGF2α+PMSG and hCG) than the G1 (control group). The recorded data were (100, 100 and 92%,
respectively). The data also indicated that conception rate was significantly higher (P<0.05) in G3 than in G2 and G1

(100, 92 and 88%). On the other hand, abortion rate was higher in control group (G1) surpassing G2 and G3, as both
groups G2 and G3 showed no abortion (9.0, 0.0 and 0.0%, respectively) (Table 1).

Table 1. Estrus response and conception and abortion rates % of Barki ewes as affected by different hormonal protocols

(LSM±SE)

Treatment (T)

Traits

Overall mean

± SEM

Significance

G1

G2

G3

92.00

100.0

100.0

97.33

Estrus response

5.30

NS

(23/25)

(25/25)

(25/25)

(73/75)

88.00

92.00

100.00

93.33

Conception rate

4.87

*

(22/25)

(23/25)

(25/25)

(70/75)

09.00

Abortion rate

00.00

00.00

(2/22)

G1 = control group; G2 = FGA and PGF2α; G3 = FGA, PGF2α, PMSG and hCG; NS = non-significant

58

To cite this paper: Ashour G, El-Bassiony MF, Dessouki ShM and El-Wakeel MA (2018). Application of Different Hormonal Protocols for Improving Reproductive Performance of Barki Ewes. World Vet. J. 8(3): 55-64.

Journal homepage: www.wvj.science-line.com

The results of this study confirm that using the intravaginal progestagen impregnated sponge is an effective tool for the synchronization of the estrus. These results were previously confirmed in goats at the beginning of the breeding
season in semi-desert conditions (Riaz et al., 2012). In our study, progesterone treatment lasted for 12 days showed high
efficiency. Hosseinipanah et al. (2014) concluded that the occurrence of estrus using CIDR in non- reproductive season
for 10, 12 and 14 days was associated with the onset of good estrus and use of GnRH had positive effect on fertility in
treatment groups, with the highest significant value for the 12 days P4 treatment. In the same year, Zoharaa et al. (2014)

recorded higher pregnancy rates as observed in the 30 mg FGA treatment (100%) compared with 100 μg Cloprostenol

(88.2 %), 175 μg Cloprostenol (75%) and 45 mg FGA (93.8 %) treatments. It was concluded that though FGA sponge
protocol presented superior results.

In comparison with our results, Abdul Muin et al. (2013) suggested that CIDR treatment for 14 days with 400 IU

PMSG and 0.05 mg cloprostenol prior to CIDR removal gave better result in estrus synchronisation than in CIDR
treatment for 9 days with 0.05 mg cloprostenol given 24 hours before CIDR removal. In the present study both treatments were high to estrous response with no significant except to the control group. But the results of this study
agree with recent report of Waheeb et al. (2017) who concluded that intravaginal progesterone sponge for 7 days+500IU
eCG at sponge removal is convenient for estrus synchronization of ewes raised in field conditions during breeding
season specially that the reproductive and fertility parameters recorded in his study were acceptable and within values
reported previously in farm conditions. Also, Dendena (2017) stated a fertility rate of 85.1% after the administration of

500 IU of eCG, which was considered lower than PMSG treatment in this study, this may be referred to using higher
dose (750 IU) in our treated group. Thus, our results were comparable to what has been most recently reported by

Fornazari et al. (2018) as these stated that short-term progestagens (FGA e MAP) + eCG were 100.0% efficient in Assaf
breed in cycle control worth to mention that in the same study the administration of 750 IU of eCG determined a fertility
rate of 76.5%, which was controversially to our results. From our point of view, less prolific and less seasonal breeds
tend to be more responsive to eCG administration, specially that different follicular populations present on the ovaries
before progestagen treatments + eCG may also condition fertility rate (Omontese et al., 2016). In general, the present
results meet these last investigators’ observations that exogenous gonadotropin administration advanced ovulation and
higher estrus synchronization precision (Letelier et al., 2011; Valentim et al., 2016), as they support ovarian mechanisms
affecting follicular growth and maturation and promoting the proper luteinization of the CL (Valentim et al., 2016). In
most studies, it is mentioned that in adult animals the expected estrus rate is greater than 90%, while nulliparous goats
can reach up to 97.2% and lactating animals up to 85.7% (Abecia et al., 2011; Navanukraw et al., 2014; Alvarado-Espino
et al., 2016).

Lambing rate, abortion rate, weaning rate, fecundity rate, litter size at birth, litter size at weaning and
mortality rates (%)

Lambing rate is expressed as the percentage of ewes lambed out of ewes mated. As shown in table 2 lambing rate
was significantly higher (P<0.05) in G3 as compared to G2 or G1 (100, 92 and 80%, respectively). While, weaning rate
was recorded to be higher in G2 than in G1 and G3 and showed non-significantly rates of 100.0, 95.45 and 94.29%,
respectively.

Table 2. Lambing rate, weaning rate, fecundity rate, litter size at birth, weaning rate, litter size at
weaning and weaning weight of Barki ewes as affected by different hormonal treatment (LSM±SE)

Treatment (T)

Traits

Overall mean

± SEM

Sig.

G1

G2

G3

80.00

92.00

100.00

90.67

Lambing rate (%)

4.63

**

(20/25)

(23/25)

(25/25)

(68/75)

95.45

100.0

94.29

Weaning rate (%)

96.39

5.95

NS

(21/22)

(26/26)

(33/35)

0.88b

1.04b

1.40a

1.10

**

Fecundity rate (%)

0.10

(22/25)

(26/25)

(35/25)

4.55

0.00

5.71

Mortality rate (%)

3.61

--

NS

(1/22)

(0/26)

(2/35)

1.10b

1.13b

1.40a

1.22

*

Litter size at birth/ewe

0.05

(22/20)

(26/23)

(35/25)

1.05

1.32

1.17

Litter size at weaning/ewe

1.13 (26/23)

0.05

NS

(21/20)

(33/25)

Day 90 (Weaning weight)(kg)

16.71

17.53

17.45

17.28

--

NS

G1 = control group; G2 = FGA and PGF2α; G3 = FGA, PGF2α, PMSG and hCG; Sig. = significance; NS = non-significant

Means within each row with different superscripts are significantly different at 5% level.

59

To cite this paper: Ashour G, El-Bassiony MF, Dessouki ShM and El-Wakeel MA (2018). Application of Different Hormonal Protocols for Improving Reproductive Performance of Barki Ewes. World Vet. J. 8(3): 55-64.

Journal homepage: www.wvj.science-line.com

As reported in our study, fecundity rate was significant higher in G3 and G2 than in G1 (1.40, 1.04 and 0.88),
respectively (Table 2). Moreover, litter size at birth as expressed as number of born lambs per ewe lambed is considered to be an important parameter of fertility. Data illustrated in table 2 indicated that litter size at birth was significantly the
highest in G3 followed by G2 as compared to G1 (1.40, 1.13 and 1.10, respectively). Litter size at weaning was
significantly higher in G3 then G2 than in G1 (1.32, 1.13 and 1.05 respectively). The data also revealed that weaning
weight showed insignificant increase in G2 as compared to G3 and G1 (17.53, 17.45 and 16.71, respectively). Non-
significant higher values were recorded for mortality rate in G3 and G1 than in G2 (5.71, 4.55 and 0.00%, respectively).

In the tropics, biological and economic efficiency of animal production are predominantly determined by
reproductive performance. Fertility is categorized as important parameters for determination the productivity of sheep;
fertility may be estimated by several indicators as litter size per lambing for example which is more important than
weight gain of lambs. Profit gain from increasing lamb survival is the major concern of sheep producers (Elliott et al.,

2011). According to Elliott et al. (2011), sheep producers believed that using teasers for estrus synchronization, minimizing handling and interrupting ewes during the peri-partum period, and limiting duration of the breeding period
would be beneficial to lamb survival. Contrarily to our results, it has been confirmed that high dose of eCG increases the
ovulation rate but also reduces the embryonic survival rate (Diskin and Morris, 2008).

Formerly Gongnet (1996) reported malnutrition as the main cause of lamb mortality before weaning in lambs, for
that reason, in recent study by Allou et al. (2013) advised with proper diet to enhance the adequate health that improve
the survivability and lambs growth during the first three months of age. While, Gbangboche et al. (2005) referred the
lamb survival rate before weaning to the age of the sheep at the first lambing influences, this was confirmed by Allou et
al. (2013), who had stated that lambs survival is positively and beneficially correlated with this influence . Early reports
indicated that the number of lambs surviving until weaning has been found to be influenced by ewes’ body condition
score at mating (Carson et al., 2001) rather than other surrounding factors.

Lambs with intermediate birth weights have a lower mortality risk than small and very large lambs (Piwczynski et
al., 2012). However, the association between lamb birth weight and mortality was not detected in a study by Brien et al.

(2009). In this regard, due to negative effects of large litters on lamb survival, close supervision should be provided to
non-singleton lambs (Chniter et al., 2011). Litter size is influenced by several factors, and is low in ewes younger than 2
years of age and ewes having low weight at mating (Atashi et al., 2013). Weight gain in lambs is described by two
subsequent phases, the first lasts from birth up to 90 days with a moderate weight change, this phase is followed by very
fast changes in weight form 90 days until 200 days (7 months) of age (Allou et al., 2013).

Progesterone concentration

The results in Figure 2 to elucidate that plasma progesterone levels (ng/ml) as affected by FGA and PGF2α (G2) and

FGA, PGF2α, PMSG and hCG (G3) as compared to the control group (G1). It could be observed that P4 levels attended
to increase from the first day of pregnancy and rises to the end of the day before parturition. Also, progesterone was
higher in G3 which contains the highest values of litter size at birth of newborn lambs as a result of increasing twinning
rates in response to hormonal treatment for the superovulation. Mean concentration of P4 level was found to be 8.47
ng/ml in G3 followed by G2 (8.05 ng/ml) while the lowest value was observed in G1 (7.40 ng/ml).

Early pregnancy is a critical period for maternal recognition, and the lack of nutrients that stimulate an increase in
circulating progesterone can increase early embryonic losses (Viñoles et al., 2012). According to Mann et al. (2006),
most embryonic losses occur during the first few days after fertilization and during the implantation process, inadequate
luteal function being one of the main causes. The maintenance of pregnancy in ruminants depends on the continued
secretion of progesterone by the corpus luteum, which inhibits luteolysis. Progesterone deficiency due to primary luteal
insufficiency has been reported as a cause of embryonic death (Mann and Lamming, 2001; Diskin and Morris, 2008).

Increase concentrations of progesterone (P4) during meta-oestrus and early di-oestrus improves embryonic growth and
the production of interferon-τ (IFN-τ) (Spencer, 2013 and 2016; Arosh et al., 2016), which in turn improves the relationship between embryo and uterus and increases embryonic survival rates (Mann et al., 2006).

The higher values of progesterone levels, found in G3 followed by G2 than that in the control group, may be due to
the number of corpora lutea present as it is the source of progesterone in sheep. El-Tarabany (2012) found that progesterone concentration decreased significantly in ewes conceived single than in ewes conceived twin's fetuses by

34.3%. Also, Khan and Ludri (2002) found that in twin bearing goats' plasma progesterone level was significantly higher
than in single bearing goats during all days of experiment. Manalu et al. (1998) reported that ewes with a high litter size
had heavier placental tissue. Moreover, ewes with a high litter size had high ovulation rates that resulted in high number
of corpora lutea as sources of progesterone during the embryonic phase of pregnancy and, probably, throughout the
pregnancy period

60

To cite this paper: Ashour G, El-Bassiony MF, Dessouki ShM and El-Wakeel MA (2018). Application of Different Hormonal Protocols for Improving Reproductive Performance of Barki Ewes. World Vet. J. 8(3): 55-64.

Journal homepage: www.wvj.science-line.com

Figure 2. Plasma progesterone levels (ng/ml) as affected by FGA and PGF2α (G2) and FGA, PGF2α, PMSG and hCG

(G3) compared to the control group (G1). G1 = control group; G2 = FGA and PGF2α; G3 = FGA, PGF2α, PMSG and hCG

Type of lambing and sex ratio

The data presented in table 3 demonstrate that single rate was significantly higher in the control non treated group

(G1) as compared to other treated groups, being 90.00, 86.96 and 64.00%, respectively. Results of present study indicated that twining rate significantly increased in the hormonal manipulated group (G3) than (G2) and (G1) being

32.00, 13.04 and 10.00%, respectively, (Table 3). The influence of the superovulation treatment was observed for G3, as
triple rate increased significantly in G3 as compared to other groups, with values of 4.0, 0.0 and 0.0%, respectively. Sex
ratio (female %) exhibited significantly the highest values for G3 (60.00%) followed by G2 (59.09%) and finally G1

(46.15%). Conversely, sex ratio (male %) was significantly higher in G2 than in G1 and G3, recording 53.85, 40.91 and

40.00%, respectively.

A like to the Barki breed in our study herein, Awassi breed is a monotoccus with an ovulation rate of 1 and very low
incidence of twinning (Jaber et al., 2004). For that reason, the twins and triplets born lambs weighed less at birth as
compared with singles in control groups, this might be due to the competition between fetuses for nutrient supplied by
the placenta from maternal circulation for growth due to the low capacity of uteri to provide for twins (Baneh and

Hafezian, 2009). In Barki, twining in general and especially triple births is not common. Moreover, Clement et al. (1997)

in his study assumed that the longer of lambing interval is accompanied with the increase in the range of sizes with the
rank of lambing. Herein this study triples birth in group 3 (G3) is probably accompanied with the effect of PMSG
injection. Sheep male lambs’ are known to be born heavier than females, (Gbangboché et al., 2005), who also reported
that this trend in weight significant different may last up to 12 months or more, this observation is reported under same
rearing conditions for birth weight for males and females, but these variations tends to disappear at 120 days of age

(Poivey et al., 1982; Fall et al., 1983). Binns et al. (2002) and Brien et al. (2009) found higher mortality in male than
female lambs. On the other hand, another study reported a higher survival risk in male lambs (Atashi et al., 2013), while
mortality risks for gender were not statistically different in a study in Quebec (Arsenault et al., 2003).

Table 3. Types of lambing and sex ratio (%) of Barki ewes as affected by different hormonal protocols

(LSM±SE)

Treatment (T)

Traits

Overall mean

± SEM

Significance

G1

G2

G3

90.00

86.96

64.00

Single rate

--

--

--

(18/20)

(20/23)

(16/25)

10.00b

13.04b

32.00 a

Twining rate

18.34

2.74

**

(2/20)

(3/23)

(8/25)

4.00a

Triple rate

0b

0b

--

--

--

(1/25)

59.09

46.15

60.00

55.42

Sex ratio (female)

1.62

*

(13/22)

(12/26)

(21/35)

(46/83)

40.91

53.85

40.00

44.58

Sex ratio (male)

1.62

*

(9/22)

(14/26)

(14/35)

(37/83)

61

To cite this paper: Ashour G, El-Bassiony MF, Dessouki ShM and El-Wakeel MA (2018). Application of Different Hormonal Protocols for Improving Reproductive Performance of Barki Ewes. World Vet. J. 8(3): 55-64.

Journal homepage: www.wvj.science-line.com

G1 = control group; G2 = FGA and PGF2α; G3 = FGA, PGF2α, PMSG and hCG. Means within each row with different superscripts are significantly different at 5% level.

CONCLUSION

It could be concluded that, hormonal manipulation using intravaginal progestagen impregnated sponge and PMSG
in the presence of hCG; for estrous synchronization and superovulation; would be a proper way for enhancing the
reproductive efficiency of Barki ewes.

Acknowledgments

The authors are thankful to Dr. Khalid El-Bahrawy for his support and assisting in lab work and guide through
writing the manuscript. Deepest thanks are due to Dr. Hamdy Gawish for facilitating the research work at the Sustainable

Development Center for Matrouh Resources. We also would like to appreciate all participants who contributed during
sample collection.

Competing interests

The authors declare that they have no conflict of interest with respect to the research, authorship, and/or publications of this article. The authors declare that they have no competing interests.

Author’s contribution

Dr. Gamal Ashour designed the experiment, article writing and revision, Dr. Moharram Fouad El-Bassiony
designed the experiment, laboratory analyses, statistical analysis, tabulation of experimental data, manuscript writing,
commenting and approval, Dr. Sherif Mohamed Dessouki helped in statistical analysis, tabulation of experimental data
and article writing; while, Mr. Mohamed Awad El-Wakeel helped in field study, collected data, laboratory analyses,
manuscript writing. All authors have read and approved the final manuscript.

REFERENCES

Abdul-Muin HB, Hasbudie B, Suraya MS, Panandam JM, Yaakub H, Theivanai J and Quaza Nizamuddin HN (2013). Effects of two

CIDR-based oestrus synchronization protocols on oestrus response in Boer goats. Malaysian Journal Animal Science, 16(2): 29-

35.

Abecia JA, Forcada F and González-Bulnes A (2011). Pharmaceutical control of reproduction in sheep and goats. Veterinary Clinics of North America: Food Animal Practice, 27(1):67–79. DOI:10.1016/j.cvfa.2010.10.001

Allou DS, Farougou S and Hountondji FCC (2013). Impact of prophylactic measures and the use of local food resources on the
viability and growth of pre-weaning lambs in Djougou, in the northern region of Benin. Animal and Plant Sciences, 19(3): 2933-

2940.

Alvarado-Espino AS, Meza-Herrera CA, Carrillo E, González-Álvarez VH, Guillen-Muñoz JM, Ángel-García O and Mellado M

(2016). Reproductive outcomes of Alpine goats primed with progesterone and treated with human chorionic gonadotropin
during the anestrus-to-estrus transition season. Animal Reproduction Science, 167:133–138.

DOI:10.1016/j.anireprosci.2016.02.019

Andriyanto A and Manalu W (2011). Increasing goat productivity through the improvement of endogenous secretion of pregnant
hormones using follicle stimulating hormone. Animal Production, 13(2): 89-93.

Arosh JA, Banu SK and McCracken JA (2016). Novel concepts on the role of prostaglandins on luteal maintenance and maternal
recognition and establishment of pregnancy in ruminants. Journal of Dairy Science, 99(7):5926–5940. DOI:10.3168/jds.2015-

10335

Arsenault J, Dubreuil P, Girard C, Simard C and Belanger D (2003). Maedi-visna impact on productivity in Quebec sheep flocks

(Canada). Preventive Veterinary Medicine, 59: 125-137. Doi:https://doi.org/10.1016/S0167-5877(03)00086-2

Atashi H, Izadifard J, Zamiri MJ and Akhlaghi A (2013). Investigation in early growth traits, litter size, and lamb survival in two Iranian fat-tailed sheep breeds. Tropical Animal Health and Production, 45: 1051-1054. DOI:10.1007/s11250-012-0308-9

Baneh H and Hafezian H (2009). Effects of environmental factors on growth traits in Ghezel sheep. African Journal of Biotechnology, 8:2903-2907. Doi:10.5897/AJB09.284

Binns SH, Cox IJ, Rizvi S and Green LE (2002). Risk factors for lamb mortality on UK sheep farms. Preventive Veterinary Medicine,

52: 287-303. Doi:https://doi.org/10.1016/S0167-5877(01)00255-0

Brien FD, Hebart ML, Jaensch KS, Smith DH and Grimson RJ (2009). Genetics of lamb survival: a study of Merino resource flocks in

South Australia. Proceedings of Association for the Advancement of Animal Breeding and Genetics, 18: 492-495.

Doi:https://www.researchgate.net/profile/Forbes_Brien

Carson AF, Irwin D and Kilpatrick DJ (2001). A comparison of Scottish Blackface and Cheviot ewes and five sire breeds in term of
lamb output at weaning in hill sheep system. The Journal of Agriculture Science, 137: 221-233.

Doi:https://doi.org/10.1017/S0021859601001277

62

To cite this paper: Ashour G, El-Bassiony MF, Dessouki ShM and El-Wakeel MA (2018). Application of Different Hormonal Protocols for Improving Reproductive Performance of Barki Ewes. World Vet. J. 8(3): 55-64.

Journal homepage: www.wvj.science-line.com

Chniter M, Hammadi M, Khorchani T, Krit R, Lahsoumi B, Sassi MB, Nowak R and Hamouda MB (2011). Phenotypic and seasonal factors influence birth weight, growth rate and lamb mortality in D’man sheep maintained under intensive management in

Tunisian oases. Small Ruminant Research, 99:166-170. Doi:https://doi.org/10.1016/j.smallrumres.2011.03.046

Chniter M, Hammadi M, Khorchani T, Sassi MB, Hamouda MB and Nowak R (2013). Aspects of neonatal physiology have an
influence on lambs’ early growth and survival in prolific D’man sheep. Small Ruminant Research, 111:162-170.

Doi:https://doi.org/10.1016/j.smallrumres.2012.10.004

Clément V, Poivey JP, Faugère O, Tillarde Lancelot R, Gueye A, Richard D and Bibe B (1997). Etude de la variabilité des caractères de reproduction chez les petits ruminants en milieu traditionnel au Sénégal. Revue d'élevage et de médecine vétérinaire des pays
tropicaux, 50:235-249.

Cognie Y, Baril G, Poulin N and Mermillod P (2003). Current status of embryo technologies in sheep and goat. Theriogenology. 59:

171-188. Doi:https://doi.org/10.1016/S0093-691X(02)01270-0

Dendena M (2017). Controlo Da Actividade Reprodutiva e Inseminação Artificial Em Ovelhas Da Raça Churra Galega Bragançana (Thesis). 53 p.

Diskin MG and Morris DG (2008). Embryonic and early foetal losses in cattle and other ruminants. Reproduction of Domestic

Animals, 43(2): 260–267. Doi:10.1111/j.1439-0531.2008.01171.x

Duncan DB (1955). Multiple range and multiple F tests. Biometrics, 11: 1- 42.

Elliott J, Sneddon J, Lee JA and Blache D (2011). Producers have a positive attitude toward improving lamb survival rates but may be influenced by enterprise factors and perceptions of control. Livestock Science, 140:103-110.

Doi:https://doi.org/10.1016/j.livsci.2011.02.015

El-Tarabany AA (2012). Physiological changes in ewes conceived single or twin’s fetuses related with survivability of lambs. Arab

Journal of Nuclear Science and Applications, 45(3): 1-12.

Fall A, Diop M, Sandford J, Gueye E, Wissocq YJ, Durkin J and Trail JCM (1983). Etude sur la productivité de moutons Djallonké au

Centre de Recherches Zootechniques de Kolda, au Sénégal: 2. Poids corporels, productivité des brebis et du troupeau. Revue
d'élevage et de médecine vétérinaire des pays tropicaux, 36(3):283-289.

Fonseca J, Bruschi J, Zambrini F, Demczuk E, Viana J and Palhão M (2005). Induction of synchronized estrus in dairy goats with
different gonadotrophins. Animal Reproduction, 2: 50-53.

Fornazari R, Mateus Ó, Correia T, Quintas H, Maurício R, Conradi A, Francisco L, Álvaro A and Valentim R (2018). Estrus synchronization and artificial insemination with fresh and chilled semen in Assaf ewes. Agriculture Science, 9:8-22.

DOI:10.4236/as.2018.91002

Gbangboche AB, Hornich JL, Adamou-N’Diaye M, Edorh AP, Fanir F, Abiola FA and Leroy PL (2005). Caractérisation et maîtrise des
paramètres de la reproduction et de la croissance des ovins Djallonké (Ovis amon aries). Annales de Médecine Vétérinaire,

149:170-182.

Gongnet GP (1996). Étude des causes de mortalité des agneaux Peul-Peul et Touabire avant sevrage dans la zone sylvo-pastorale au

Sénégal. Sciences Vétérinaires et Médecine Comparée, 98:133-42.

Hosseinipanah SM, Anvarian M, Mousavinia M, Alimardan M, Hamzei S and Zengir SBM (2014). Effects of progesterone in
synchronization of estrus and fertility in Shal ewes in nonproductive season. European Journal of Experimental Biology, 4(1):83-86.

Husein MQ, Ababneh MM and Haddad SG (2005). The effects of progesterone priming on reproductive performance of GnRH-PGF2
alpha-treated anestrous goats. Reprod. Nutr. Dev., 45:689-698. DOI: 10.1051/rnd:2005053

Jaber LS, Habre A and Rawda N (2004). The effect of water restriction on certain physiological parameters in Awassi sheep. Small

Ruminant Research, 54:115-120. Doi:https://doi.org/10.1016/j.smallrumres.2003.11.004

Jainudeen M, Wahid H and Hafez E (2000). Ovulation induction, embryo production and transfer. In: Reproduction in Farm Animals,

7th Edition. Lippincott Williams & Wilkins. Maryland. USA. 405-430.

Kaya A, G ـNes E and Memili E (2018). Application of reproductive biotechnologies for sustainable production of livestock in Turkey.

Turkish Journal of Veterinary and Animal Sciences, 42: 143-151. Doi:10.3906/vet-1706-66

Kearl LC (1982). Nutrient Requirements of Ruminants in Developing Countries. Utah Agric. Exp. Sta. Utah State University, Logan,

U S A.

Khan JR and Ludri RS (2002). Hormonal profiles during periparturient period in single and twin fetus bearing goats. Asian-Australian Journal of Animal Science, 15(3):346-351. Doi:https://doi.org/10.5713/ajas.2002.346

Koyuncu M and Alticekic SO (2010). Effects of progestagen and PMSG on estrous synchronization and fertility in Kivircik ewes
during natural breeding season. Asian-Australian Journal of Animal Science, 23:308-311.

Doi:https://doi.org/10.5713/ajas.2010.90393

Kusina N, Tarwirei F, Hamudikuwanda H, Agumba G and Mukwena J (2000). A comparison of the effects of progesterone sponges
and ear implants, PGF2alpha, and their combination on efficacy of estrus synchronization and fertility of Mashona goat does.

Theriogenology, 53:1567-1580. Doi:https://doi.org/10.1016/S0093-691X(00)00298-3

Letelier CA, Contreras-Solis I, Garcia-Fernandez RA, Sanchez MA, Garcia-Palencia P, Sanchez B, Ariznavarreta C, Tresguerres JAF,

Flores JM and Gonzalez-Bulnes A (2011). Effects of oestrus induction with progestagens or prostaglandin analogues on ovarian
and pituitary function in sheep. Animal Reproduction Science, 126:61-69. Doi:https://doi.org/10.1016/j.anireprosci.2011.04.012

Manalu W, Sumaryadi MY, Sudjatmogo and Satyaningtijas AS (1998). Effect of superovulation on maternal serum progesterone
concentration, uterine and fetal weights at weeks 7 and 15 of pregnancy in Javanese thin-tail ewes. Small Ruminant Research,

30:171–176. Doi:https://doi.org/10.1016/S0921-4488(98)00121-7

63

To cite this paper: Ashour G, El-Bassiony MF, Dessouki ShM and El-Wakeel MA (2018). Application of Different Hormonal Protocols for Improving Reproductive Performance of Barki Ewes. World Vet. J. 8(3): 55-64.

Journal homepage: www.wvj.science-line.com

Mann GE and Lamming GE (2001). Relationship between maternal endocrine environment, early embryo development and inhibition of the luteolytic mechanism in cows. Reproduction, 121(1):175–180. Doi:https://www.ncbi.nlm.nih.gov/pubmed/11226041

Mann GE, Fray MD and Lamming GE (2006). Effects of time of progesterone supplementation on embryo development and interferon-τ production in the cow. Veterinary Journal, 171(3):500–503. DOI: 10.1016/j.tvjl.2004.12.005

Mekuriaw Z, Assefa H, Tegegne A and Muluneh D (2016). Estrus response and fertility of Menz and crossbred ewes to single
prostaglandin injection protocol. Tropical Animal Health and Production, 48:53-57. DOI:10.1007/s11250-015-0919-z

Motlomelo K, Greyling J and Schwalbach L (2002). Synchronisation of oestrus in goats: the use of different progestagen treatments.

Small Ruminant Research, 45:45-49. DOI:10.1016/S0921-4488(02)00113-X

Navanukraw C, Khanthusaeng V, Kraisoon A and Uriyapongson S (2014). Estrous and ovulatory responses following cervical artificial insemination in Thainative goats given a new or once-used controlled internal drug release with human chorionic
gonadotropin. Tropical Animal Health and Production, 46 (8):1441–1446. DOI:10.1007/s11250-014-0662-x

Omontese BO, Rekwot PI, Ate IU, Ayo JO, Kawu MU, Rwuaan JS, Nwannenna AI, Mustapha RA and Bello AA (2016). An update on
oestrus synchronisation of goats in Nigeria. Asian Pacific Journal of Reproduction, 5: 96-101.

Doi:https://doi.org/10.1016/j.apjr.2016.01.002

Piwczynski D, Sitkowska B and Wisniewska E (2012). Application of classification trees and logistic regression to determine factors responsible for lamb mortality. Small Ruminant Research, 103:225-231. Doi:https://doi.org/10.1016/j.smallrumres.2011.09.014

Poivey JP, Landais E and Berger Y (1982). Etude et amélioration génétique de la croissance des agneaux Djallonké Résultats obtenus
au Centre de Recherches Zootechniques de Bouaké (Côte-d'Ivoire). Revue d’élevage et médecine vétérinaire des pays tropicaux, 35(4):421-433. Doi:https://agritrop.cirad.fr/455195/1/ID455195.pdf

Riaz H, Sattar A, Arshad MA and Ahmad N (2012). Effect of synchronization protocols and GnRH treatment on the reproductive
performance in goats. Small Ruminant Research, 104(1):151–155. Doi:https://doi.org/10.1016/j.smallrumres.2011.10.008

SAS (2004). Statistical analysis system User's Guide. Release 9.1. SAS institute, Cary, North Carolina, USA.

Souza A, Garcia-Guerra A and Adams G (2014). Advances in ovarian stimulation. In: Proceedings of the 2014 AETA and CETA/ACTE Joint Convention; Madison, WI, USA.

Spencer TE (2013). Early pregnancy: concepts, challenges, and potential solutions. Animal Frontiers, 3(4):48–55.

Spencer TE, Forde N and Lonergan P (2016). The role of progesterone and conceptus-derived factors in uterine biology during early
pregnancy in ruminants. Journal of Dairy Science, 99(7):5941–5950. Doi:https://doi.org/10.3168/jds.2015-10070

Valentim R, Rodrigues I, Montenegro T, Sacoto S, Azevedo J and Gomes MJ (2016). Artificial Insemination in Sheep and Goat. In:

Reproduction Management in Sheep and Goat. Agrotec. Portugal. ISSN 2182-4401. 21:10-13.

Viñoles C, Glover KMM, Paganoni BL, Milton JTB and Martin GB (2012). Embryo losses in sheep during short-term nutritional
supplementation. Reproduction and Fertility Development, 24(8):1040–1047. Doi:http://dx.doi.org/10.1071/RD11281

Waheeb RS, El-amrawi GA, Metwelly KK and El-sabbagh AM (2017). Synchronization of estrus in field conditions using progestagen sponge, GnRH, and PGF2α in Barki ewes during breeding season. Alexandria Veterinary Science, 54(2): 1-4.

DOI:10.5455/ajvs.273830

Webb R, Garnsworthy P, Gong JG and Armstrong D (2004). Control of follicular growth: local interactions and nutritional influences.

Journal of Animal Science, 82: E63-E74. DOI:10.2527/2004.8213_supplE63x

Yavuzer Ü (2005). The possibilities of twice-yearly lambing of Awassi sheep ewes without using hormones in an organic animal
production system. Turkish Journal of Veterinary Animal Science, 29:27-30.

Zarkawi M (2001). Oestrous synchronisation and twinning rate of Syrian Awassi ewes treated with progestagen and PMSG during the
breeding

season.

New

Zealand

Journal

of

Agricultural

Research,

44:

159-163.

Doi:https://doi.org/10.1080/00288233.2001.9513472

Zohara BF, Azizunnesa, Islama F, Alam GS and Bari FY (2014). Comparison of estrus synchronization by PGF2α and progestagen
sponge with PMSG in indigenous ewes in Bangladesh. GSTF International Journal of Veterinary Science, l (1):27-37.

Doi:http://dl6.globalstf.org/index.php/JVet/article/view/1330/1248

64

To cite this paper: Ashour G, El-Bassiony MF, Dessouki ShM and El-Wakeel MA (2018). Application of Different Hormonal Protocols for Improving Reproductive Performance of Barki Ewes. World Vet. J. 8(3): 55-64.

Journal homepage: www.wvj.science-line.com