Scienceline Publications World'€™s Veterinary Journal 2322-4568 10 2 2020 June Butanol Fraction of Kelussia odoratissima Mozaff Inhibits the Growth of Leishmania major Promastigote and Amastigote 254 259 http://wvj.science-line.com/attachments/article/61/WVJ%2010(2)%20254-259,%20June%2025,%202020.pdf EN Farzaneh Mirzaei Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Roghayeh Norouzi Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Abolghasem Siyadatpanah Ferdows School of Paramedical and Health, Birjand University of medical sciences, Birjand, Iran Watcharapong Mitsuwan School of Allied Health Sciences, World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si Thammarat, Thailand Mohammadali Nilforoushzadeh Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran Iran; Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Amir Maleksabet Department of Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran Mohsen Hosseini Department of Biostatistics and Epidemiology, School of Public Health, IUMS, Isfahan, Iran Maria de Lourdes Pereira 0CICECO-Aveiro Institute of Materials and Department of Medical Sciences, University of Aveiro, Aveiro, Portugal Veeranoot Nissapatorn School of Allied Health Sciences, World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si Thammarat, Thailand Seyed Hossein Hejazi Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Naturally derived compounds have been used to treat several infectious diseases including leishmaniasis. The study aimed to investigate the in vitro effects of Kelussia odoratissima Mozaff extract on Leishmania major promastigote and amastigote. Dried leaves of K. odoratissima were fractionated by 3 solvents including aqueous, butanol, and ethyl acetate. The results showed that the butanol fraction of K. odoratissima showed the highest anti-Leishmania effects against L. major promastigotes. Ninety four percent growth inhibition of the promastigote was observed when cells were treated with the 1,280 µg/mL butanol fractions. Moreover, 100% inhibition of amastigotes was detected after treatment with the butanol fraction. Half maximal inhibitory concentration (IC50) of the butanol fraction in promastigotes and amastigotes was 264.1 and 154.1 µg/mL, respectively. The obtained results suggested the potential medicinal benefits of K. odoratissima butanol fraction as an alternative treatment for leishmaniasis caused by L. major infections. Amastigotes Butanol fraction Leishmania major Kelussia odoratissima Mozaff Promastigotes